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Image Search Results
Journal: bioRxiv
Article Title: Tumor-Intrinsic Response to IFNγ Shapes the Tumor Microenvironment and Anti-PD-1 Response in NSCLC
doi: 10.1101/531236
Figure Lengend Snippet: LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), CD8 + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
Article Snippet: Antibodies and Reagents: 89Y-CD45, Fluidigm, Clone 30-F11; 141Pr-Gr1 (Ly6C/Ly6G), Fluidigm, Clone RB6-8C5; 142Nd-CD11c, Fluidigm, Clone N418; 143Nd-GITR, Fluidigm, Clone DTA1; 144Nd-MHC class I, Fluidigm, Clone 28-14-8; 145Nd-SiglecF-PE/anti-PE, BD, Clone E50-2440/ Fluidigm, Clone PE001;
Techniques: Injection, Isolation, Flow Cytometry, Staining, Immunofluorescence, Expressing
Journal: Cancer Cell
Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity
doi: 10.1016/j.ccell.2021.06.017
Figure Lengend Snippet:
Article Snippet: Anti-PE 145Nd clone PE001 ,
Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry