ecl plus hypersensitive luminescent solution pe0010 Search Results


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LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
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LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
3156005b Cd74 Ln2 Fluidigm Er, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson 145nd-siglecf-pe/anti-pe, e50-2440/fluidigm, pe001
LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
145nd Siglecf Pe/Anti Pe, E50 2440/Fluidigm, Pe001, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ecl Pe001, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), CD8 + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Journal: bioRxiv

Article Title: Tumor-Intrinsic Response to IFNγ Shapes the Tumor Microenvironment and Anti-PD-1 Response in NSCLC

doi: 10.1101/531236

Figure Lengend Snippet: LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), CD8 + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Article Snippet: Antibodies and Reagents: 89Y-CD45, Fluidigm, Clone 30-F11; 141Pr-Gr1 (Ly6C/Ly6G), Fluidigm, Clone RB6-8C5; 142Nd-CD11c, Fluidigm, Clone N418; 143Nd-GITR, Fluidigm, Clone DTA1; 144Nd-MHC class I, Fluidigm, Clone 28-14-8; 145Nd-SiglecF-PE/anti-PE, BD, Clone E50-2440/ Fluidigm, Clone PE001; 146Nd-CD8a, Fluidigm, Clone 53-6.7; 147Nd-p-H2AX[Ser139], Fluidigm, Clone JBW301; 148Nd-CD11b, Fluidigm, Clone M1/70; 149Sm-CD19, Fluidigm, Clone 6D5; 150Nd-CD25, Fluidigm, Clone3C7; 151Eu-CD64, Fluidigm, Clone X54-5/7.1; 152Sm-CD3e, Fluidigm, Clone 145-2C11; 153Eu-PD-L1, Fluidigm, Clone 10F.9G2; 154Sm-CTLA4, Fluidigm, Clone UC10-4B9; 155Gd-IRF4, Fluidigm, Clone 3E4; 156Gd-CD90.2(Thy-1.2), Fluidigm, Clone 30-H12; 158Gd-FoxP3, Fluidigm, Clone FJK-16s; 159Tb-PD-1, Fluidigm, Clone RMP1-30; 160Gd-CD80/86-FITC/anti-FITC, BD, Clone 16-10A1/BD Clone BL1/Fluidigm, Clone FIT22; 161Dy-INOS, Fluidigm, Clone 4B10; 162Dy-Tim3, Fluidigm, Clone RMT3-23; 163Dy-CXCR3-APC/anti-APCCXCR3-173, Biologend/Fluidigm, Clone APC003; 164Dy-IkBa, Fluidigm, Clone L35A5;165Ho-Beta-catenin (active), Fluidigm, Clone D13A1; 166Er-Arg1, Fluidigm, Clone 6D5; 167Er-NKp46, 2 Fluidigm, Clone 9A1.4; 168Er-Ki-67; Fluidigm, Clone Ki-67; 169Tm-Ly-6A/E (Sca-1), Fluidigm, Clone D7; 170Er-CD103-Biotin/anti-Biotin, Biolegend, Clone 2E7/Fluidigm, Clone 1D4-C5; 171Yb-CD44, Fluidigm, Clone IM7; 172Yb-CD4, Fluidigm, Clone RM4-5; 173Yb-CD117 (ckit), Fluidigm, Clone 2B8; 174Yb-Lag3, Fluidigm, Clone M5/114.15.2;175Lu-CD127, Fluidigm, Clone A7R34; 176Yb-ICOS, Fluidigm, Clone 7E.17G9; 191Ir, 193Ir-Intercalator, Cell-ID; 195Pt Cisplatin 5 uM, Cell-ID; 140Ce, 151Eu, 153 Eu, 165Ho, 175Lu Normalization Beads; Cell ID 20-Plex Pd Barcoding Kit, Fluidigm,#201060 (102, 104, 105, 106, 108, 110 Pd Bar Codes); Benzonase, Sigma #E1014-5KU(1:10,000) in HBSS.

Techniques: Injection, Isolation, Flow Cytometry, Staining, Immunofluorescence, Expressing

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-PE 145Nd clone PE001 , Fluidigm , 3145006B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry